Column:

Chiralomix^® SB( 5 µm, 4.6 x 250 mm )

Mobile phase:

N-hexane : IPA: methanol : diethylamine = 90 : 5 : 5 : 0.1( v/v )

Flow rate:

1.0 mL/min

Temperature:

10℃

Detection:

UV 230 nm

Injection volume:

20 µL

Sample:

1.0 mg/mL (D)L-tryptophan methyl ester hydrochloride by IPA, then diluted 5 times by N-hexane